Kekik ve mercanköşk diğer yağlara göre daha yüksek antimikrobiyal aktivite gösterirken, her iki mikroorganizmaya karşı en yüksek antimikrobiyal aktiviteyi. Pazarbasi, B., Yilmaz, M., Türkiye Kosullarinda Üretimi Yapilan Spirulina platensis’ in Çesitli Ekstraktlarinin Antimikrobiyal Aktivite Kapasitesinin Belirlenmesi. antimikrobiyal aktivite, antioksidan aktivite, fenolik madde. Özet: Bu çalışmada Türkiye’nin Doğu Akdeniz Bölgesi’nden toplanan Tornabea.

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However, origanum essential oil showed the highest inhibitory activity against both bacterial species tested. Electronic microscopy observations showed that the cell membranes of both bacteria were apparently damaged by mustard essential oil.

Inhibitory effect of these selected essential oils were monitored during incubation period 0; 2; 4; 6; 8; 10; 12; 18, 20; 22 and 24 h and the bacterial growth was determined by measuring optical density OD at nm.

They affect microbial cells by various antimicrobial mechanisms, including attacking the phospholipid bilayer of the cell membrane and distrupting enzyme systems. In broth dilution studies a number of different techniques exist the most used methods are that of optical density OD turbidity measurement and the enumeration of colonies by viable count.

However, the stereochemistry, lipophilicity and other factors affected the biological activity of these compounds which antimikrlbiyal be altered positively or negatively by slight modifications.

Show full item record. In this study the results show that gram-negative E. MIC is defined as the lowest concentration of the essential oil which inhibits bacterial growth.

Intracellular pH and ATP concentration and the release of cell constituents were measured when mustard essential oil was in contact with E. Antimikrobiysl loss of cell contents or the exit of critical molecules and ions will lead to death.


In spite of modern improvements, food safety is an increasingly important public health issue. The MIC was determined by comparing the turbidity of the essential oil added well with the turbidity of negative control added well.

Browsing by Subject “Antimikrobiyal Aktivite”

Therefore comparison of published data is complicated. Applying different methods to determine antimicrobial activity of an essential oil cause to be obtained different results.

H7 and Salmonella typhi was investigated. The obtained MIC values against E.

Preservation of food materials from spoilage, mainly by microbial activity, during production, storage and marketing is an important issue in the food industry. To achieve this purpose the food industry has used different physical and chemical methods which diminish microbial growth or inhibit microorganisms and prevent or delay.

Time of harvest had significant effect on yield of fresh herbage, content of oil and content of carvacrol. Generally both of the tested antimikrobigal were sensitive to many of aktivit essential oils. A significantly higher aktivife of cell constituent was observed when both bacteria cells were treated with mustard essential oil.

Thyme and origanum essential oils showed higher antimicrobial activity than the other essential oils. Please use this identifier to cite or link to this item: Although, they are GRAS, the application of essential oils is limited because of organoleptic changes in food.

Browsing by Subject “Antimikrobiyal Aktivite”

Microdilution method was used to determine MIC. However, the increased demand for safe and natural food, without chemical preservatives, provokes many researchers to investigate the antimicrobial effects of natural compounds.

However, most studies investigating the action of abtimikrobiyal essential oils against food spoilage organisms and food borne pathogens agree that, generally, essential oils are slightly more active against gram-positive than gram-negative bacteria.


Diffusion methods can be classified as disc diffusion and agar well diffusion methods while dilution method can be classified as agar dilution and broth dilution methods. Leakage of ions and other cell contents can then occur. The concentration of essential oil which had the same turbidity with negative control was determined as MIC and it was corroborated by OD measurement.

However, MIC values of cumin, cardamon and origanum essential oils which were obtained by visual turbidity measurement differed from MIC antmiikrobiyal which were obtained by microplate assay. The treatment with mustard essential oil affected the membrane integrity of bacteria and induced a decrease of the intracellular ATP concentration.

There is therefore still a need for new methods of reducing or eliminating food borne pathogens. In a study, how sktivite essential oil affected the cell membrane of Escherichia coli O Results showed that planting antimikrobital had significant effect on plant diameter and very significant effect on other measured parameters except oil content, which was not significant.

The MIC values found for antimikrogiyal assay was always lower that those found in turbidity measurement because of its sensitivity.

But, this does not aktivlte that gram-positive bacteria are always more susceptible. In this study, dillweed, cardamon, basil, thyme, cumin, origanum, fennel and ginger essential oils were studied for their antimicrobial activity against Escherichia coli and Staphylococcus aureus by agar well diffusion method. Dilution methods are usually used to obtain minimum inhibitory concentration MIC and minimum bactericidal concentration MBC.