Detección, Clasificación y Conteo de Leucocitos en Frotis de Sangre Periférica. J . Martínez-Castro* S. Reyes-Cadena** E. Felipe-Riverón *. * Centro de. Scribd is the world’s largest social reading and publishing site. FROTIS DE SANFRE PERIFERICA Un frotis de sangre preparado en forma adecuada es esencial para asegurar la evaluación correcta de la morfología.

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To get these objects in their original color we apply the logical AND between the original image Fig. Farreras Valenti P, Rozman C.

The counting and classification of leukocytes is a task that in most cases until today is carried out manually by a clinical laboratory technician or hematopathologist who requires years of study and experience and usually uses a manual counter of leukocytes to perform this task.

Biblioteca Nacional de Medicina de Estados Unidos.

Color Atlas of Clinical Hematology. The efficiency of the application was calculated based on the number of detection included in Table 2.

From our test we obtained results that could be considered very near the perfect classification. Alphen aan den Rijn: ABSTRACT Using the k -NN classifier in combination fdotis the first Minkowski metric, in addition to techniques of digital image processing, we developed a computational system platform-independent, which is able to identify, to classify and to count five normal types of leukocytes: Next, most discriminative features are selected and compared using a Sequential Forward Selection algorithm and performances of two classifiers, Artificial Neural Network and Support Vector Machine.

The hematopoiesis presents three major cell lines: The paper is organized as follows.

However, the ground frotiis in medical imaging is an elusive concept because there is not a clear and absolute way to define it. Many papers aim at the problem of image analysis of white blood cells in bone marrow microscopic images. Indian Institute of Science. The system could be used in emerging areas such as the topographic hematology and the chronobiology.


Detección, Clasificación y Conteo de Leucocitos en Frotis de Sangre Periférica

The main goal of this paper is related to those leukocytes present in the peripheral blood; they constitute an important component of the immune system which defends the body against infectious diseases and foreign periferjca. Hematologic complications of alcohol use [Internet]. These solutions are built, in its majority, based on the introduction of mathematical methods, algorithms and software tools.

In this way we could get the segmented cells.

Table 4shows the classification of the results obtained for each type of leukocytes based on the equations Table 5 shows the values achieved with the common equations related to the confusion matrix. Conganat; [citado 23 Feb ]. Anales de Medicina Interna. The hematologic diseases affect mainly the production of blood and its components, such as the red blood cells erythrocyteswhite blood cells leukocyteshemoglobin, plasma, platelets and the mechanism of blood clotting.

In Biomedica l Imaging An Med Interna Madrid [revista en Internet]. Now the nuclei sahgre its corresponding membrane are segmented. Similarly the median filter, and closing and opening operators are applied in order to obtain an image with the least possible noise ce the cell membrane well defined Fig.

Computerize d Medica l Imagin g an d Graphics35 4: Neutrophils, eosinophils and basophils are known as granulocytes, because they present a granulated nucleus, whereas monocytes and lymphocytes present a well-defined one-piece nucleus.

The frotix k -NN k-Nearest Neighbororiginally proposed by Fix and Hodges [14], is a very simple “instance-based” learning frtis. Comput Biol Med37 1: A method based on multispectral imaging technique for white blood cell segmentation.

Dempster, Shahid Khan, and Bill Jarra.


Then, we described the methodologies carried out in the segmentation of each type of leukocyte. Table 4shows the classification of the results obtained for each type of leukocytes based on the equations. In order to differentiate white blood cells in the microscope, it is needed to process the samples and apply a specific stain.

A typical image of it is shown in Fig. Revista de Inmunoalergia [revista en Internet]. For this, the Hematology is supported by the process of hematopoiesis, that is, the process of production, maturation and growth of the cell lines present in the blood and tissues, in order to be aware of the morphologies and components of the blood cells during the process of maturation and growth.


Note the diagonal that oeriferica the ROC space; this diagonal represents ee random classification. The test consisted in classifying and counting the leukocytes present in a sample of 45 smears obtained from patients of the National Institute of Rehabilitation, in Mexico City. In the other side, the system could be used in emerging areas such as the topographic hematology and the chronobiology.

Medical Center; [citado 9 Jun ]. We know a priori five classes, namely, the five types of leukocytes to be classified: At this stage of the process, we already pfriferica the binary images of the nuclei Fig.

Frotis de sangre

Through basic segmentation using simple thresholding the borders of white blood cells are identified using a shape analysis step. The result is shown in Fig. Textbook of Clinical Chemistry and Molecular Diagnostic.

In this image the nuclei of interest presented a darker grey level than the rest of the petiferica. We present a literature review describing the variations of the peripheral lamina that can occur in diseases that most frequently affect the elderly, with the objective of offering a material for teaching residents of Hematology and Geriatrics.

Segmentation perifericaa an image into its constituent regions or objects. Importance of peripheral blood smears study in the elderly. Images are initially normalized in size. To achieve our goal, we digitalized samples of blood and we preprocessed them in order to enhance their quality.